Thermo Fisher Scientific Goat anti-Canine IgG (H+L) Secondary Antibody, HRP
개 IgG(H+L)에 특이적인 HRP 결합 2차 항체로 Western blot, IHC, ELISA에 적합합니다. 염소에서 유래된 폴리클로날 항체이며 항원 친화 크로마토그래피로 정제되었습니다. 높은 민감도와 다양한 검출 시스템에 활용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,500 |
| ELISA | 1:50,000–1:150,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Dog IgG from normal dog serum |
| Conjugate | HRP |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15 M NaCl |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_10978282 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Formats
- [AP]
- [Biotin]
- [FITC]
- [Request custom conjugation]
Product Specific Information
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Anti-Canine secondary antibodies are affinity-purified antibodies with well-characterized specificity for canine (dog) immunoglobulins and are useful in detection, sorting, or purification of the specified target.
Secondary antibodies offer increased versatility, enabling users to use many detection systems (e.g., HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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