Thermo Fisher Scientific Complement C3 Polyclonal Antibody
Goat polyclonal antibody specific to human complement C3. Recognizes C3 fragments (C3a, C3b, C3c). Validated for WB, IHC, ICC/IF, Flow, and IE. Supplied as liquid, 90 mg/mL, stored at 4°C or -20°C. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 6 publications |
| Immunohistochemistry (IHC) | – | View 3 publications |
| Immunocytochemistry (ICC/IF) | 1:500 | View 1 publication |
| Flow Cytometry (Flow) | 1:100 | – |
| Immunoelectrophoresis (IE) | Assay-dependent | – |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Baboon, Horse, Guinea pig, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat |
| Published Species | Cynomolgus monkey, Guinea pig, Human, Mouse, Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Human complement C3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 90 mg/mL |
| Storage Buffer | 0.05 M Tris-HCl, pH 7.5, with 500 mM NaCl |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4 °C for short term. For long-term storage, store at −20 °C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2066730 |
Product Specific Information
- Specificity: Monospecific to human C3 complement component.
- Recognizes C3 fragments: C3a, C3b, and C3c.
- Supplied as diluted unpurified antiserum.
- Store as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
C3 is a key component of the complement system, where classical and alternative activation pathways merge at the C3 activation step when C3 is split into C3a and C3b.
The molecular mass of C3 is approximately 185 kDa and consists of two chains (110 kDa and 75 kDa) linked by disulfide bonds.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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