Thermo Fisher Scientific SKA3 Polyclonal Antibody
Rabbit polyclonal antibody targeting human SKA3 protein. Validated for WB, IHC(P), and IP applications. Supports research on mitotic spindle and kinetochore complex function. For research use only, not for diagnostic purposes.
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Thermo Fisher Scientific SKA3 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunoprecipitation (IP) | 1–5 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived Human SKA3 fragment |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
Target Information
Upon entry into mitosis, the cell’s microtubule (MT) network forms the mitotic spindle, enabling chromosome segregation. Kinetochores (KT) on centromeric chromatin are essential for proper attachment of chromosomes to spindle MTs. The spindle and kinetochore complex (SKA), composed of SKA1, SKA2, and SKA3, is required for stable KT-MT interactions and timely anaphase onset.
Depletion of SKA3 by siRNA delays anaphase transition, resulting in a prolonged metaphase-like state. SKA3-depleted cells accumulate high levels of checkpoint protein Bub1 at kinetochores, indicating that the SKA complex is crucial for spindle checkpoint silencing and chromosome cohesion maintenance during mitosis.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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