
Thermo Fisher Scientific EpCAM/CD326 Recombinant Rabbit Monoclonal Antibody (EGP40, 4546R)
EpCAM/CD326에 특이적인 재조합 토끼 단클론 항체로, 인체 조직의 면역조직화학(IHC) 분석에 적합합니다. HEK293 발현 시스템에서 생산되었으며, Protein A/G 정제 및 PBS 버퍼에 보존됩니다. Ep-CAM 발현 종양 연구에 활용 가능합니다.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | EGP40, 4546R |
| Immunogen | Recombinant full-length human Ep-CAM protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed):
Incubate antibody for 30 min at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in epithelial tissues (~40 kDa). It functions as a Ca²⁺-independent homotypic cell adhesion molecule, influencing cell cycle, proliferation, and metabolism via induction of proto-oncogene c-myc and cyclins A/E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression is linked to enhanced epithelial proliferation and is highly expressed in human carcinomas, serving as a marker for epithelial lineage tumors.
Ep-CAM is found on the basolateral surface of most simple epithelia and many carcinoma types, and can distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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