Thermo Fisher Scientific HCoV-HKU1 Spike S2 Polyclonal Antibody
HCoV-HKU1 Spike S2 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 ELISA에 적합합니다. 고순도 친화 크로마토그래피로 정제되었으며, -20°C 보관 시 안정적입니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Virus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 800–900 of coronavirus Spike S2 (YP_1732381) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.75 mg/mL |
| Purification | Affinity Chromatography |
| Storage buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2914341 |
Product Specific Information
- Positive test controls include: Human coronavirus HKU1 (isolate N5) (HCoV-HKU1) Spike Protein (S1+S2 ECDHis Tag).
- Immunogen sequence:
IVGQEEFIQT NSPKVTIDCS LFVCSNYAAC HDLLSEYGTF CDNINSILDE VNGLLDTTQL HVADTLMQGV TLSSNLNTNL HFDVDNINFK SLVGCLGPHC G
Target Information
The S2 Spike protein mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. [Uniprot]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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