Thermo Fisher Scientific HCoV-OC43 Spike S2 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000

ELISA

• Tested Dilution: 1 µg/mL

Product Specifications

Product Specific Information

Positive test controls include: Human coronavirus (HCoV-OC43) Spike Protein (S1+S2 ECDHis Tag).

Immunogen sequence:
EPVGGLYEIQ IPSEFTIGNM VEFIQTSSPK VTIDCAAFVC GDYAACKSQL VEYGSFCDNI NAILTEVNEL LDTTQLQVAN SLMNGVTLST KLKDGVNFNV

Target Information

The S2 Spike protein mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide near the C-terminal region of the ectodomain. The formation of this structure drives apposition and subsequent fusion of viral and target cell membranes. [Uniprot]

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.