Thermo Fisher Scientific HCoV-OC43 Spike S2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Virus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 782-881 of coronavirus Spike S2 (YP_0095552411)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.95 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914343

Product Specific Information

Positive test controls include: Human coronavirus (HCoV-OC43) Spike Protein (S1+S2 ECDHis Tag).

Immunogen sequence: EPVGGLYEIQ IPSEFTIGNM VEFIQTSSPK VTIDCAAFVC GDYAACKSQL VEYGSFCDNI NAILTEVNEL LDTTQLQVAN SLMNGVTLST KLKDGVNFNV

Target Information

The S2 Spike protein mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. [Uniprot]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.