Thermo Fisher Scientific HIF1AN Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:1,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Protein corresonding to Human HIF1AN. Recombinant protein control fragment (Product #RP-103593). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HIF1AN,uniProtId:Q9NWT6-1,ncbiNodeId:9606,antigenRange:80-158,antigenLength:349,antigenImageFileName:PA5-111599_HIF1AN_Q9NWT6-1_Rabbit.svg,antigenImageFileNamePDP:PA5-111599_HIF1AN_Q9NWT6-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2857009

Product Specific Information

Immunogen sequence: YLQENIGNGD FSVYSASTHK FLYYDEKKMA NFQNFKPRSN REEMKFHEFV EKLQDIQQRG GEERLYLQQT LNDTVGRKI

Target Information

Hydroxylates HIF-1 alpha at Asn-803 in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.