Thermo Fisher Scientific HIF1AN Polyclonal Antibody
Human HIF1AN 단백질을 인식하는 Rabbit Polyclonal 항체로, IHC(P)에서 1:500~1:1,000 희석 비율로 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS/glycerol buffer에 보관. HIF-1α 산소 감지 및 전사 조절 연구에 적합.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P)): 1:500–1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Protein corresponding to Human HIF1AN. Recombinant protein control fragment (Product # RP-103593) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2857009 |
Product Specific Information
Immunogen sequence:
YLQENIGNGD FSVYSASTHK FLYYDEKKMA NFQNFKPRSN REEMKFHEFV EKLQDIQQRG GEERLYLQQT LNDTVGRKI
Target Information
Hydroxylates HIF-1 alpha at Asn-803 in the C-terminal transactivation domain (CAD).
Functions as an oxygen sensor; under normoxic conditions, hydroxylation prevents interaction of HIF-1 with transcriptional coactivators (Cbp/p300-interacting transactivator).
Involved in transcriptional repression through interaction with HIF1A, VHL, and histone deacetylases.
Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A, and others.
Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively.
Negatively regulates NOTCH1 activity (myogenic differentiation) and positively regulates ASB4 activity (vascular differentiation).
[UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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