Thermo Fisher Scientific RbBP5 Polyclonal Antibody
RbBP5 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF, IP, ChIP 등 다양한 응용에 적합합니다. 1 mg/mL 액상 형태이며 4°C에서 보관합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:250–1:1,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
| ChIP assay (ChIP) | see datasheet |
| ChIP-sequencing (ChIP-Seq) | 2 µg |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 500 and the C-terminus (residue 538) of Human retinoblastoma binding protein 5 (RBBP5, UniProt ID: Q15291-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
This gene encodes a ubiquitously expressed nuclear protein that belongs to a highly conserved subfamily of WD-repeat proteins. The encoded protein binds directly to retinoblastoma protein, which regulates cell proliferation. It interacts preferentially with the underphosphorylated retinoblastoma protein via the E1A-binding pocket B. Three alternatively spliced transcript variants encoding different protein isoforms have been described for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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