Thermo Fisher Scientific CD8a Monoclonal Antibody (RPA-T8), eFluor 506, eBioscience

Applications

Immunohistochemistry (IHC)

• Tested Dilution: Not specified
• View 1 publication

Flow Cytometry (Flow)

• Tested Dilution: 5 µL (0.125 µg)/test
• View 38 publications

Product Specifications

Additional Available Formats

• Alexa Fluor 532, 561, 660, 700
• APC, APC-eFluor 780
• Brilliant UV 395–805
• Brilliant Violet 421–786
• eFluor 450, FITC
• NovaFluor Blue, Red, Yellow series
• PE, PE-Cyanine5/5.5/7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436–780
• Request custom conjugation

Product Specific Information

The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, a 32–34 kDa cell surface receptor expressed as CD8α/β heterodimers or CD8α/α homodimers. It is expressed on most thymocytes, a subset of mature T cells, and NK cells. CD8 binds MHC class I molecules and, through association with protein tyrosine kinase p56lck, plays a key role in T-cell development and activation.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for flow cytometry of normal human peripheral blood cells. Recommended usage: 5 µL (0.125 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Fluorochrome Information:
eFluor® 506 is excited with a 405 nm violet laser and emits at 506 nm. Use a 510/20 bandpass filter or equivalent.
Excitation: 405 nm
Emission: 506 nm
Laser: Violet
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family. It functions as a co-receptor with TCR for MHC class I molecules and plays a critical role in T cell differentiation, activation, and signal transduction. CD8 is expressed on cytotoxic T cells, NK cells, monocytes, and dendritic cells as CD8α/β heterodimers or CD8α/α homodimers. Upon MHC-I/peptide binding, CD8 recruits Lck kinase, leading to cytokine production and cytotoxic T lymphocyte (CTL) activation. Activated CTLs are essential for eliminating pathogens and tumor cells. Differentiation of naïve CD8⁺ T cells into CTLs is promoted by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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