Thermo Fisher Scientific Smoothened homolog Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200

-

ELISA (ELISA)

1:500-1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide derived from human Smoothened, amino acids 551-650. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SMO,uniProtId:Q99835-1,ncbiNodeId:9606,antigenRange:551-650,antigenLength:787,antigenImageFileName:BS-2801R_SMO_Q99835-1_Rabbit.svg,antigenImageFileNamePDP:BS-2801R_SMO_Q99835-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol

Contains

0.02% ProClin 300

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

Smoothened drosophila homolog (SMOH) is a serpentine protein belonging to the G-protein coupled receptor Fz/Smo family with 787 aa, seven transmembrane G protein-coupled receptors with 4 glycosylation sites and a putative extracellular amino terminus. Smoothened activated by b-Arrestin 2 and GRK2 acts as a receptor for Shh, the Sonic hedgehog gene product, and is a positive regulator of hedgehog signaling pathway along with the smoothened inhibitor patched protein (PTCH) and regulates vertebrate developmental processes, cellular proliferation and differentiation by activating transcription factor Gli. Smoothened mutants are known to cause sporadic basal cell carcinoma of the skin. It is known to express widely in heart and brain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.