Thermo Fisher Scientific HSP60 (Heat Shock Protein 60) Recombinant Mouse Monoclonal Antibody (rGROEL, 780)
Recombinant mouse monoclonal antibody targeting HSP60 for WB, IHC, and peptide array applications. Reacts with human and mouse, expressed in HEK293 cells. High purity, liquid form, suitable for mitochondrial protein studies.
- 카탈로그번호
- 3329-MSM7-P0
- 판매단위
- pk
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Applications
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 0.25–0.5 µg/mL | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL | |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rGROEL, 780 |
| Immunogen | Recombinant full-length human HSP60 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- This antibody does not react with bacteria, Helminths, or Spinach.
- For immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system and is essential for the folding and assembly of newly imported proteins in the mitochondria. The gene is adjacent to a related family member, and the region between the two genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified. Mutations in this gene cause autosomal recessive spastic paraplegia 13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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