Invitrogen™

Galacto-_Star_™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells

The Galacto-Star™ One-Step β-galactosidase Reporter Gene Assay System chemiluminescent reporter gene assay system enables rapid, sensitive detection of β-galactosidase in자세히 알아보기

The Galacto-Star™ One-Step β-galactosidase Reporter Gene Assay System chemiluminescent reporter gene assay system enables rapid, sensitive detection of β-galactosidase in mammalian cell lysates.

• Glow assay kinetics provides a “window” during which measurements may be performed, facilitating HTS applications where assay automation is used.
• Can be used with either mammalian or yeast model systems, providing flexibility in choice of model systems.
• Wide dynamic range of β-galactosidase assay lets the user measure enzyme level accurately from femtogram to nanogram range.
• Assay sensitivity is 100- to 1,000-fold better than either the isotopic⁄non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric⁄fluorescent assays for β-galactosidase, providing greater sensitivity than competing assays.
• Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions.
• Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes.
• Assay can be completed in about one hour, providing fast assay turnaround.

Sensitive
The chemiluminescent assay exhibits over three orders of magnitude greater sensitivity than colorimetric β-galactosidase assays and can be completed in a fraction of the time required for assays for chloramphenicol acetyltransferase (CAT) and ELISA-based reporter assays.

Versatile
The Galacto-Star™ system includes Galacton-Star™ chemiluminescent substrate for β-galactosidase and is suited for measurement in both luminometers and scintillation counters. Automatic injection is not required.

Long-Lasting Signal
Light emission from the Galacto-Star™ assay reaches maximum in 60-90 minutes and remains constant for nearly one hour. Cell lysate is mixed with reaction buffer containing Galacton-Star™ substrate and Sapphire-II™ luminescence enhancer. After a 60-90 minute incubation at room temperature, the signal is measured in a luminometer or scintillation counter.

For Research Use Only. Not for use in diagnostics procedures.

사양

제품 유형β-Galactosidase Reporter Gene Assay System

검출 방법Chemiluminescence

용도(장비)Luminometer (Microplate)

어세이β-Gal (lacZ) Assay

호환 셀Mammalian Cells

고처리량 호환성High-throughput Compatible

라벨 또는 염료Unconjugated

판독End Point

샘플 종류Cell Cultures

기질Galacton-Star™

기질 유형Beta-Gal Substrate

기질 특성Chemical Substrate

타겟 효소Beta-Galactosidase

제품라인Galacto-Star™, NovaBright™

용도(애플리케이션)Enzyme Assay

수량600 Assays

형태Liquid

Unit Size600 assays