Thermo Fisher Scientific AFP Polyclonal Antibody
인간 AFP를 인식하는 Rabbit Polyclonal Antibody로 Western blot, IHC, ICC, ELISA 등 다양한 응용에 사용 가능. Protein A로 정제된 액상 항체이며, -20°C에서 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human AFP, amino acids 521–609 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
AFP (Alpha Fetoprotein) is a major plasma protein produced by the yolk sac, intestinal tract, and liver during fetal life. In adults, AFP expression is often associated with hepatoma or teratoma, though hereditary persistence may occur without pathology. AFP is the fetal counterpart of serum albumin, with both genes located tandemly on chromosome 4. It exists in monomeric, dimeric, and trimeric forms and can bind copper, nickel, fatty acids, and bilirubin. AFP levels in amniotic fluid are used to screen for neural tube defects such as spina bifida and anencephaly. Elevated serum AFP is observed in hepatocellular carcinoma, teratoblastoma, colorectal, pancreatic, and germ cell tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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