Thermo Fisher Scientific p130Cas Polyclonal Antibody
Western blot, IHC, ICC 등 다양한 응용에 적합한 p130Cas 폴리클로날 항체. Human, Mouse, Rat 반응성. 항원 친화 크로마토그래피로 정제된 고순도 제품. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 250–710 of Human BCAR1 (p130Cas) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.379 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2544701 |
Product Specific Information
- Recommended positive controls: H1299, NIH-3T3, 293T, p130Cas shRNA-transfected 293T
- Predicted reactivity: Mouse (88%), Rat (88%), Bovine (88%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The p130Cas (Cas for Crk-associated substrate) is a common cellular target of phosphorylation signaling via v-Crk and v-Src oncoproteins.
It has a unique structure containing a Src homology (SH)-3 domain followed by multiple YXXP motifs and a proline-rich region.
p130Cas is implicated in various biological processes including cell adhesion, migration, growth factor stimulation, cytokine receptor engagement, and bacterial infection.
Physiological functions include cardiovascular development, actin filament assembly, and Src-induced cell transformation.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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