Thermo Fisher Scientific CD258 (LIGHT) Monoclonal Antibody (7-3 (7)), PE, eBioscience

Thermo Fisher Scientific CD258 (LIGHT) Monoclonal Antibody (7-3 (7)), PE, eBioscience

Applications

• Reported Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.25 µg)/test
• Publications: View 2 publications

Product Specifications

Additional Formats

• APC
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This 7-3 (7) monoclonal antibody reacts with human CD258 (LIGHT), a 29-kDa type II transmembrane protein belonging to the TNF ligand superfamily related to lymphotoxin-β (LT-β).
LIGHT is expressed on activated T cells, especially CD8+ T cells, and plays roles in cell survival, inflammation, and tumor eradication. Upon binding to its ligand HVEM, LIGHT induces a costimulatory effect on T cells, enhancing proliferation and cytokine production. LIGHT can also interact indirectly with the LT-β receptor.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and validated on PMA- and ionomycin-stimulated normal human peripheral blood cells
  • Recommended use: 5 µL (0.25 µg)/test (100 µL final volume)
  • Cell number: 10⁵–10⁸ cells/test (empirically determined)
• Excitation: 488–561 nm
• Emission: 578 nm
• Compatible Lasers: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Apoptosis, or programmed cell death, is a key process in cellular differentiation and development. It is induced by cytokines such as TNF and Fas ligand through their death domain-containing receptors (TNFR1 and Fas).
Signals are transduced by adapter molecules and proteases of the ICE/CED-3 family. A novel DD-containing molecule, Daxx (death associated protein 6), binds specifically to the Fas death domain, enhancing Fas-induced apoptosis and activating the JNK pathway. Daxx is widely expressed in human and mouse tissues, indicating its critical role in Fas signaling.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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