Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (eBioJ105 (J105)), PE, eBioscience

Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (eBioJ105 (J105)), PE, eBioscience™

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 5 µL (0.5 µg)/test
  View 30 publications

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 615, 661, 737, 805
• Brilliant Violet 421, 480, 650, 711, 786
• PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The J105 monoclonal antibody reacts with human PD-1 (programmed death-1), a 55 kDa member of the CD28 immunoglobulin superfamily. PD-1 contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. PD-1 is expressed predominantly on activated T and B lymphocytes.
Two ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC), have been identified, showing overlapping functions and expression patterns.

Costaining experiments suggest eBioJ105 recognizes a different epitope than MIH4 (Product #11-9969).

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated on PHA-stimulated human peripheral blood cells

• Recommended use: 5 µL (0.5 µg)/test
• A test = antibody amount for staining 100 µL cell sample (10⁵–10⁸ cells/test)

Excitation: 488–561 nm
Emission: 578 nm
Compatible lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

PD-1 (PDCD-1) is an inhibitory receptor expressed on activated T cells that binds PD-L1 and PD-L2 on antigen-presenting cells to regulate immune activation. Binding of PD-1 to its ligands inhibits T-cell activation in the absence of danger signals such as LPS. PD-1 deficiency leads to hyperactivated immune responses and autoimmune disease.
Despite its predicted molecular weight, PD-1 often migrates at a higher apparent molecular weight in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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