Thermo Fisher Scientific CTGF Polyclonal Antibody, PeproTech
Thermo Fisher Scientific의 CTGF Polyclonal Antibody는 인간 CTGF 단백질을 검출하기 위한 고순도 항체로, Western blot과 ELISA에 최적화되어 있습니다. 항원 친화 크로마토그래피로 정제되었으며, 인간·마우스·랫에 반응합니다. 연구용으로만 사용됩니다.
- 카탈로그번호
- 500-P252-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CTGF Polyclonal Antibody, PeproTech
Thermo Fisher Scientific CTGF Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 1 publication |
| ELISA | 0.5–2.0 µg/mL | View 1 publication |
| Immunoprecipitation (IP) | – | View 1 publication |
| Neutralization (Neu) | – | View 1 publication |
| In vitro Assay (IV) | – | View 1 publication |
| Miscellaneous PubMed (Misc) | – | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human CTGF |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930073 |
Product Specific Information
AA Sequence of recombinant protein:
GKKCIRTPKI SKPIKFELSG CTSMKTYRAK FCGVCTDGRC CTPHRTTTLP VEFKCPDGEV MKKNMMFIKT CACHYNCPGD NDIFESLYYR KMYGDMA
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human CTGF. Anti-Human CTGF-specific antibody was purified by affinity chromatography employing an immobilized Human CTGF matrix.
Sandwich ELISA:
To detect Human CTGF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human CTGF (500-P252Bt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human CTGF.
Western Blot:
To detect Human CTGF by Western Blot analysis, use at a concentration of 0.1–0.2 µg/mL. With compatible secondary reagents, the detection limit for Recombinant Human CTGF is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
Connective Tissue Growth Factor (CTGF), also called CCN2, is a member of the CCN family.
CTGF is a secreted protein produced by umbilical veins and vascular endothelial cells.
It possesses an Insulin-like Growth Factor (IGF)-binding domain, a thrombospondin type 1 domain, and a cysteine knot region.
CTGF plays important roles in chondrocyte proliferation and differentiation, induces angiogenesis, and promotes cell adhesion in fibroblasts, endothelial, and epithelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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