Thermo Fisher Scientific CD127 Monoclonal Antibody (A7R34), NovaFluor Red 700, eBioscience

Thermo Fisher Scientific CD127 Monoclonal Antibody (A7R34), NovaFluor Red 700, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1.0 µg/test

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Product Specifications

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Available Formats

• Unconjugated
• Alexa Fluor 488, 561, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 661, 737, 805
• Brilliant Violet 421, 650, 711
• eFluor 450, 660
• FITC
• Functional Grade
• NovaFluor Red 710
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 645
• Request custom conjugation

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Product Specific Information

The A7R34 monoclonal antibody reacts with mouse CD127, the high affinity alpha subunit of the mouse IL-7 receptor.
CD127 is expressed by immature B cells in bone marrow, single-positive thymocytes (CD4+ or CD8+), but not double-positive thymocytes. Mature T cells express CD127 at low levels.

A7R34 inhibits IL-7 binding to its receptor and is widely used in in vivo and in vitro studies of IL-7 function in T and B cell development and activation.
Binding of A7R34 blocks SB/199 antibody binding to mouse CD127.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse thymocytes and splenocytes
Recommended use ≤ 1.0 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
Careful titration is recommended for optimal assay performance.

Compatibility Notice:
NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for NovaFluor dyes.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) must be used for optimal staining.
Add 5 µL per sample before combining with cells.
For single-color controls: add 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, providing engineered fluorescent signatures optimized for minimal spillover and spread.

Excitation: 639 nm
Emission: 700 nm
Laser: 633–640 nm (Red)

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Target Information

CD127 (Interleukin-7 receptor alpha, IL-7Rα)

• Glycoprotein regulating lymphopoiesis
• Active receptor: alpha/gamma chain heterodimer
• Gamma(c) chain activates signal transduction; alpha chain mediates specific signaling
• Promotes proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+/CD8+ T cells
• Associated diseases: severe combined immunodeficiency (SCID), T⁻B⁻NK⁺ SCID

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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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