Invitrogen™

4X Bolt™ LDS Sample Buffer

Bolt LDS Sample Buffer (4X) is used to prepare protein samples for denaturing gel electrophoresis with Bis-Tris Plus gels. It자세히 알아보기

Bolt LDS Sample Buffer (4X) is used to prepare protein samples for denaturing gel electrophoresis with Bis-Tris Plus gels. It contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.

See all available buffers and reagents available for SDS-PAGE

Bolt LDS Sample Buffer contains Coomassie G250 and Phenol Red as tracking dyes instead of bromophenol blue. Coomassie G250 gives a sharp dye front with both MES and MOPS SDS running buffers and migrates much closer to the moving ion front than bromophenol blue. Bromophenol blue runs more slowly than some peptides with MES SDS Running Buffer. This ensures that small peptides do not run off the gels.

To use: Heat the sample in a 1X dilution (reduced or non-reduced) at 70°C for 10 minutes for optimal results.

Note: Bolt LDS Sample Buffer should be brought to room temperature (25°C) prior to use. It is a highly viscous and concentrated solution containing twice the amount of LDS compared to the amount of SDS in typical sample buffers. Bolt LDS Sample Buffer also contains a higher concentration glycerol.

사양

버퍼Sample Loading Buffers

농도4X

용도 (장비)Bolt Mini Gel Tank

젤 유형Bis-Tris Plus

제품 유형LDS Sample Buffer

젤 호환성Bolt™ Bis-Tris Plus Gels

제품라인Bolt™

수량10 mL

Unit SizeEach