Thermo Fisher Scientific XBP1 Monoclonal Antibody (1C4)

Applications and Tested Dilution

Product Specifications

Product Specific Information

MA5-15626 targets XBP1 in indirect ELISA and WB applications and shows reactivity with Human samples.
The immunogen is a purified recombinant fragment of human XBP1 expressed in E. coli.
MA5-15626 detects XBP1, which has a predicted molecular weight of approximately 28 kDa.

Target Information

XBP1 encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box.
This gene product is a bZIP protein that also acts as a cellular transcription factor binding to an enhancer in the promoter of T cell leukemia virus type 1.
It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator.
Upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism mediated by the endonuclease IRE1.
The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and produces the isoform XBP1(S), the functionally active transcription factor.
The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed and thought to function as a negative feedback regulator of XBP1(S), shutting off transcription of target genes during the recovery phase of ER stress.
A pseudogene of XBP1 has been identified and localized to chromosome 5.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.