Takara Straightforward RNA isolation: CellAmp direct RNA prep kit for qPCR or protein analysis

Overview

• Easy RNA isolation protocol—prepare template from cultured animal cells in only 10 minutes
• Prepared RNA can be used for one- or two-step qRT-PCR
• Works with green intercalating dye assays and probe assays
• Gene expression analysis can be completed in ~2 hours

More Information

Applications

• RNA template preparation from cultured animal cells for qPCR

Product citations

Kato, Y. et al. A lentiviral vector encoding two fluorescent proteins enables imaging of adenoviral infection via adenovirus-encoded miRNAs in single living cells. J. Biochem. 147:63–71 (2010).

Nemoto, T. et al. Role of urocortin 2 secreted by the pituitary in the stress-induced suppression of luteinizing hormone secretion in rats. Am. J. Physiol. Endocrinol. Metab. 299:E567–E575 (2010).

Nishitsuji, K. et al. Apolipoprotein E regulates the integrity of tight junctions in an isoform-dependent manner in an in vitro blood-brain barrier model. J. Biol. Chem. 286:17536–17542 (2011).

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.