Thermo Fisher Scientific RNA Polymerase II Monoclonal Antibody (CTD4H8)
RNA Polymerase II 단백질 검출용 단클론 항체로, Western blot, IHC, ICC, Flow cytometry 등에 사용 가능. Human, Mouse, Rat 반응성. CTD4H8 클론, phospho-Ser5 synthetic peptide 면역원 사용. 연구용으로만 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CTD4H8 |
| Immunogen | Ten repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
RNA polymerase II is the enzyme responsible for the synthesis of mRNA and many non-coding RNAs.
POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II.
The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence YSPTSPS.
Phosphorylation and other modifications within this sequence influence the recruitment of factors that regulate the transcription cycle.
Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the CTD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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