Thermo Fisher Scientific LEF1 Polyclonal Antibody
LEF1 단백질을 인식하는 Rabbit Polyclonal Antibody로, 인간, 마우스, 랫트 시료에 반응합니다. WB, IHC, ICC/IF에 적합하며, 고순도의 Affinity chromatography로 정제되었습니다. Wnt 신호전달 관련 연구에 유용한 연구용 항체입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human LEF1 (Accession Q9UJU2), corresponding to amino acid residues T265–A315. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2852239 |
Product Specific Information
Antibody detects endogenous levels of total LEF1.
Target Information
The LEF1 gene encodes a transcription factor belonging to a family of proteins sharing homology with high mobility group protein-1.
This protein binds to a key site in the T-cell receptor-alpha enhancer, conferring maximal enhancer activity.
It participates in the Wnt signaling pathway and may function in hair cell differentiation and follicle morphogenesis.
Mutations have been associated with somatic sebaceous tumors and other cancers, including androgen-independent prostate cancer.
Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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