Thermo Fisher Scientific APP Monoclonal Antibody (OTI3E3), TrueMAB
인간 APP 단백질을 인식하는 OTI3E3 클론의 마우스 단일클론 항체. Western blot, IHC, ICC/IF, Flow cytometry에 사용 가능. 무보존제, 동결건조 형태로 제공되며 재구성 및 결합 실험에 적합. 알츠하이머 연구에 유용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3E3 |
| Immunogen | Full length human recombinant protein of human APP produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Amyloid Precursor Protein (APP) is a cell surface kinesin I membrane receptor that mediates axonal transport of beta-secretase and presenilin 1. It is crucial for neurite growth, neuronal adhesion, and axonogenesis. APP is a 100–140 kDa transmembrane glycoprotein with multiple isoforms generated by alternative splicing. Proteolytic cleavage by beta- and gamma-secretases produces beta amyloid, the main component of senile plaques—key histopathologic features of Alzheimer’s disease. Abnormal APP processing is also linked to Down’s syndrome, early-onset familial Alzheimer’s disease, and cerebral hemorrhage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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