Thermo Fisher Scientific HSF1 Monoclonal Antibody (10H8)

Applications and Tested Dilutions

Product Specifications

Additional Formats Available

• APC
• FITC
• PE
• PerCP
• Request custom conjugation

Product Specific Information

1 µg/mL of MA5-27688 was sufficient for detection of HSF1 in 20 µg of heat-shocked HeLa cell lysate by ECL immunoblot using Goat anti-rat IgG: HRP as secondary antibody.
Detects approximately 85 kDa (unstressed cell lysates) and approximately 95 kDa (heat-shocked cell lysates).

Target Information

All organisms respond to elevated temperatures and environmental stresses by rapid synthesis of heat shock RNAs and proteins.
The regulation of heat shock gene transcription is mediated by heat shock factor (HSF), which binds to heat shock response elements (HSEs).
These HSEs are conserved across species and consist of three repeats of a 5-nucleotide {nGAAn} module arranged in alternating orientation upstream of heat shock genes.

Two HSFs have been identified in human cells: HSF1 and HSF2, sharing 38% sequence identity and binding to the same HSEs.

• HSF1 responds to classical stress signals such as heat, heavy metals, and oxidative reagents.
• HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells.

HSF1 exists constitutively in the cytoplasm and nucleus as a monomer lacking DNA-binding activity. Upon stress, HSF1 forms a nuclear-localized trimer that binds DNA.
Phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.