Thermo Fisher Scientific HSF1 Monoclonal Antibody (10H8)
HSF1 단백질을 검출하기 위한 Thermo Fisher Scientific의 Rat 모노클로날 항체(10H8). Western blot, IHC, ICC/IF 등 다양한 응용 가능. 비결합형 액상 형태로 제공되며, 고순도 Protein G 정제. 열충격 관련 스트레스 반응 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:1,000 | |
| Immunocytochemistry (ICC/IF) | 1:200 | |
| ELISA | Assay-dependent | |
| Immunoprecipitation (IP) | Assay-dependent | |
| Gel Shift (GS) | Assay-dependent |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Bovine, Guinea pig, Hamster, Human, Mouse, Rabbit, Rhesus monkey, Rat |
| Published Species | Human |
| Host / Isotype | Rat / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10H8 |
| Immunogen | Purified recombinant mouse HSF1 protein (epitope mapping to amino acids 378–395) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2735220 |
Additional Formats Available
Product Specific Information
1 µg/mL of MA5-27688 was sufficient for detection of HSF1 in 20 µg of heat-shocked HeLa cell lysate by ECL immunoblot using Goat anti-rat IgG: HRP as secondary antibody.
Detects approximately 85 kDa (unstressed cell lysates) and approximately 95 kDa (heat-shocked cell lysates).
Target Information
All organisms respond to elevated temperatures and environmental stresses by rapid synthesis of heat shock RNAs and proteins.
The regulation of heat shock gene transcription is mediated by heat shock factor (HSF), which binds to heat shock response elements (HSEs).
These HSEs are conserved across species and consist of three repeats of a 5-nucleotide {nGAAn} module arranged in alternating orientation upstream of heat shock genes.
Two HSFs have been identified in human cells: HSF1 and HSF2, sharing 38% sequence identity and binding to the same HSEs.
- HSF1 responds to classical stress signals such as heat, heavy metals, and oxidative reagents.
- HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells.
HSF1 exists constitutively in the cytoplasm and nucleus as a monomer lacking DNA-binding activity. Upon stress, HSF1 forms a nuclear-localized trimer that binds DNA.
Phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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