Thermo Fisher Scientific FGF2 Polyclonal Antibody
Rabbit polyclonal antibody against FGF2 for WB and IHC applications. Recognizes human, mouse, and rat FGF2. Lyophilized form, reconstitutable in sterile water. Suitable for research use in studying cell proliferation, angiogenesis, and tissue development.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:300–1:2,000 | - |
| Immunohistochemistry (IHC) | 1:300–1:2,000 | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide from aa region 205–255 of human basic FGF conjugated to a carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4 °C short term. For long-term storage, store at −20 °C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 150 µL of sterile water.
- Centrifuge to remove any insoluble material.
- The peptide is homologous in rat, mouse, and zebrafish.
- Specificity: FGF2.
Target Information
FGF2 (fibroblast growth factor basic) is a member of the FGF family that binds to high-affinity transmembrane receptors to regulate cell proliferation and tissue neovascularization.
It exists as five isoforms with distinct intracellular localizations and functions:
- 18 kDa isoform: cytosolic, acts via surface receptors
- 22, 22.5, 24, and 34 kDa isoforms: nuclear, may signal independently of receptors
Located on chromosome 4q in humans, FGF2 is involved in limb and nervous system development, wound healing, and tumor growth.
Different isoforms arise from alternative translation initiation at AUG and non-AUG codons, producing nuclear and cytosolic variants with distinct biological roles.
FGF2 dysfunction is associated with diseases such as Kaposi Sarcoma and corneal neovascularization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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