Thermo Fisher Scientific CD3 Monoclonal Antibody (ZM45), MonoMab

Thermo Fisher Scientific CD3 Monoclonal Antibody (ZM45), MonoMab

Applications

• Immunohistochemistry (Paraffin) (IHC (P))

Tested Dilution: Ready-to-use (150–200 µL)
Publications: Refer to Thermo Fisher Scientific documentation.

Product Specifications

Product Specific Information

This product is diluted and ready-to-use.
Recommended positive control tissue: Lymph Node (other tissues may also be suitable).

The antibody is intended for laboratory professional use in detecting the corresponding protein in formalin-fixed, paraffin-embedded tissue for qualitative immunohistochemistry (IHC) testing.
It reacts with the intracytoplasmic portion of the CD3 antigen expressed by T cells, staining both cortex and medulla of the thymus and peripheral lymphoid tissues. Suitable for normal and neoplastic T cell staining in FFPE tissues.

A synthetic 13-mer peptide corresponding to amino acids 156–168 of the epsilon chain of human CD3 protein was used as immunogen.
Pretreatment of deparaffinized tissue with heat-induced epitope retrieval or enzymatic retrieval is recommended.

IHC staining visualizes antigens through sequential application of:

1. Primary antibody to the antigen
2. Secondary (link) antibody
3. Enzyme complex
4. Chromogenic substrate

The enzymatic activation of the chromogen yields a visible product at the antigen site, interpreted under a light microscope.

Control Recommendations

• A positive tissue control must be included with every staining procedure.
• Positive controls should contain both positive and negative staining regions.
• External positive control materials should be freshly prepared and processed similarly to patient samples.
• Tissues with weak positive staining are ideal for detecting minor reagent degradation.
• Negative control tissues (internal or external) should show no specific staining, ensuring assay validity.

Target Information

The CD3 subunit complex is essential for transducing antigen-recognition signals into T cells and regulating TCR expression.
It includes CD3 gamma, delta, epsilon, and zeta subunits—members of the immunoglobulin superfamily encoded on human chromosome 11.
These subunits associate with signal transduction molecules via a double tyrosine-based motif.
CD3 plays roles in TCR-induced growth arrest, survival, and proliferation.

Expression profile:

• 68–82% of normal peripheral blood lymphocytes
• 65–85% of thymocytes
• Present in Purkinje cells of the cerebellum
• Not expressed on B or NK cells

Decreased T lymphocyte percentages may occur in autoimmune diseases.
Defects in CD3 genes are associated with CD3 immunodeficiency.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.