Thermo Fisher Scientific TIMP1 Monoclonal Antibody (F31 P2 A5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250-1:1,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

-

Immunocytochemistry (ICC/IF)

1-2 µg/mL

View 1 publication 1 publication

ChIP assay (ChIP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rhesus monkey

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

F31 P2 A5

Immunogen

Synthetic peptide corresponding to residues F(72) Q A L G D A A D I R(82) of mouse TIMP-1. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TIMP1,uniProtId:P12032-1,ncbiNodeId:10090,antigenRange:72-82,antigenLength:205,antigenImageFileName:MA1-773_TIMP1_P12032-1_House_mouse.svg,antigenImageFileNamePDP:MA1-773_TIMP1_P12032-1_House_mouse_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_889482

Product Specific Information

MA1-773 contains 100 µg of in vitro produced, protein A purified antibody (1 mg/mL) in PBS containing 1 mg/mL BSA and 0.05% sodium azide.

MA1-773 detects TIMP-1 from human samples.

MA1-773 has been successfully used in Western blot, immunofluorescence, and immunohistochemistry procedures. By Western blot, MA1-773 detects a ~30 kDa band representing TIMP-1 from K562 cell lysate. It is recommended to block with 3% BSA.

The MA1-773 immunogen is a synthetic peptide corresponding to residues F(72) Q A L G D A A D I R(82) of mouse TIMP-1.This sequence is 100% conserved in some non-human primate TIMP-1.

Target Information

TIMP1 (metalloproteinase inhibitor 1) is a metalloproteinase inhibitor that functions by forms one-to-one complexes with target metallproteinases, such as collagenases, and irreversible inactivates them by binding to their catalytic zinc cofactor. TIMP1 acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP14, and MMP16. It also functions as a growth factor that regulates cell differenation, migration, and cell death. TIMP1 activates cellular signaling cascades via CD63 and ITGB1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.