Thermo Fisher Scientific CD22 Monoclonal Antibody (MYG13)

Applications and Tested Dilution

Product Specifications

Product Specific Information

MA1-7635 detects CD22 from human, rat and mouse samples.
It has been successfully used in ELISA, flow cytometry, immunoprecipitation, and immunohistochemistry procedures.
The immunogen is a CD22 protein.

Target Information

CD22 (BL-CAM) is a type 1 integral membrane glycoprotein with molecular weight of 130–140 kDa.
It is expressed in both the cytoplasm and cell membrane of B-lymphocytes.
CD22 antigen appears early in B-cell lymphocyte differentiation at approximately the same stage as CD19 antigen.
CD22 membrane expression is limited to late differentiation stages between mature B cells (CD22+) and plasma cells (CD22−), and may be useful in phenotyping mature leukemias.
It is strongly expressed in hairy cell leukemia and also positive in diffuse large B-cell lymphoma and nodular lymphocyte predominance Hodgkin’s lymphoma, but negative in classical Hodgkin’s lymphoma.
CD22 preferentially binds to α2,6-linked sialic acid, and its recognition site can be masked by cis interactions on the same cell surface.
Upon ligand-induced tyrosine phosphorylation, CD22 is involved in regulation of B-cell antigen receptor signaling, acting both as a positive regulator through Src family kinases and as an inhibitory receptor by recruiting cytoplasmic phosphatases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.