Thermo Fisher Scientific HRI Polyclonal Antibody
HRI 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF에 사용 가능. Human EIF2AK1의 500–600번 잔기 유래 합성 펩타이드로 면역화. 액상 형태, 1 mg/mL 농도, -20°C 이하 보관. 연구용으로만 사용.
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Thermo Fisher Scientific HRI Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide conjugated to KLH derived from within residues 500–600 of Human EIF2AK1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_10986307 |
Product Specific Information
- Heat-mediated antigen retrieval recommended prior to tissue staining.
- This antibody is predicted to react with cynomolgus monkey based on sequence homology.
Target Information
EIF2AK1 (HRI) is one of four kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to environmental stresses, leading to a decrease in protein synthesis.
In the case of EIF2AK1, signaling is initiated by low levels of heme.
The HRI gene is localized to 7p22, where its 3′ end slightly overlaps with the 3′ end of the gene JTV1.
The two genes are transcribed from opposite strands, and kinase activity is induced by low levels of heme and inhibited by the presence of heme.
Usage Notice
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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