Thermo Fisher Scientific SGK1 Polyclonal Antibody
SGK1 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC, ELISA 등에 사용 가능. Human, Mouse, Rat에 반응하며, 세포막·세포질·핵 등에서 발현 검출에 적합. Affinity Chromatography 정제, 1.5 mg/mL 농도, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 172–431 of human SGK1 (NP_005618.2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804383 |
Product Specific Information
Positive Samples: HeLa, Jurkat, 293T, HepG2, Mouse kidney, Rat ovary, Rat thymus
Cellular Location: Cell membrane, Cytoplasm, Endoplasmic reticulum membrane, Mitochondrion, Nucleus
Immunogen sequence:
LYFVLDYING GELFYHLQRE RCFLEPRARF YAAEIASALG YLHSLNIVYR DLKPENILLD SQGHIVLTDF GLCKENIEHN STTSTFCGTP EYLAPEVLHK QPYDRTVDWW CLGAVLYEML YGLPPFYSRN TAEMYDNILN KPLQLKPNIT NSARHLLEGL LQKDRTKRLG AKDDFMEIKS HVFFSLINWD DLINKKITPP FNPNVSGPND LRHFDPEFTE EPVPNSIGKS PDSVLVTASV KEAAEAFLGF SYAPPTDSFL
Target Information
SGK (SGK1) is a serine/threonine kinase that may be a transcriptional target for p38/MAPK in hepatoma cells. SGK plays an important role in cellular stress response and cell survival. SGK1 is transcriptionally activated by numerous stimuli, including serum, glucocorticoids, osmotic stress, and hormones. It phosphorylates and negatively regulates pro-apoptotic FOXO3A. SGK1 is thought to play an essential function in nephropathy and cardiovascular development.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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