Thermo Fisher Scientific eIF3g Polyclonal Antibody
eIF3g 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC(P), ICC/IF에 사용 가능. 인체 시료에 반응하며 항원 친화 크로마토그래피로 정제됨. 단백질 합성 개시 복합체 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human EIF3G. Recombinant protein control fragment (Product #RP-99099) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2790738 |
Product Specific Information
Immunogen sequence:
ICKGDHWTTR CPYKDTLGPM QKELAEQLGL STGEKEKLPG ELEPVQATQN KTGKYVPPSL RDGASRRGES MQPN
Target Information
The eIF3g protein is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is essential for multiple steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi, and eIF-5 to form the 43S preinitiation complex (43S PIC). It promotes mRNA recruitment and scanning for AUG recognition, and is involved in ribosomal disassembly and recycling, preventing premature subunit joining. This subunit can bind 18S rRNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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