Thermo Fisher Scientific CPI-17 Polyclonal Antibody
CPI-17 단백질을 인식하는 토끼 폴리클로날 항체로 Western blot 및 IHC(P) 실험에 적합. 항원 친화 크로마토그래피로 정제되었으며 PBS 버퍼와 글리세롤 함유. 단기 4°C, 장기 -20°C 보관 권장. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human PPP1R14A. Recombinant protein control fragment (Product #RP-102920). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2790743 |
Product Specific Information
Immunogen sequence:
VKYDRRELQR RLDVEKWIDG RLEELYRGME ADMPDEINID ELLELESEEE RSR
Target Information
CPI-17 is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphate. It was originally identified as a PKC-potentiated inhibitory protein of protein phosphatase-1, dominantly expressed in smooth muscle. Phosphorylation at Threonine 38 by PKC or Rho-kinase enhances inhibitory potency toward myosin phosphatase. CPI-17 is also phosphorylated at Threonine 38 by protein kinase N and may be involved in calcium sensitization of smooth muscle contraction as a downstream effector of Rho and/or arachidonic acid. It is dually phosphorylated at Serine 12 and Threonine 38 by a MYPT-associated kinase (M110 kinase).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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