Thermo Fisher Scientific IL-13 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MPVPRSVSLP LTLKELIEEL SNITQDQTPL CNGSMVWSVD LAAGGFCVAL DSLTNISNCN AIYRTQRILH GLCNRKAPTT VSSLPDTKIE VAHFITKLLS YTKQLFRHGP F

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-13. Anti-Murine IL-13-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect mIL-13 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine IL-13 (500-P178) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant mIL-13.

Western Blot:
To detect mIL-13 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant mIL-13 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Package Information:
500-P178BT-1MG will be provided as 2 × 500 µg.

Target Information

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines. It plays a role in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5, and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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