Thermo Fisher Scientific IL-3 beta Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
MISDRGSDAH HLLRTLDCRT IALEILVKLP YPQVSGLNNS DDKANLRNST LRRVNLDEFL KSQEEFDSQD TTDIKSKLQK LKCCIPAAAS DSVLPGVYNK DLDDFKKKLR FYVIHLKDLQ PVSVSRPPQP TSSSDNFRPM TVEC.

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Rat IL-3β. Anti-Rat IL-3β-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Rat IL-3β by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat IL-3β (500-P177G) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Rat IL-3β.

Western Blot:
To detect Rat IL-3β by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Rat IL-3β is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Package:
500-P177GBT-1MG will be provided as 2 × 500 µg.

Target Information

IL-3 is a hematopoietic growth factor that promotes the survival, differentiation, and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil, and mast cell lineages. Produced by T cells, mast cells, and eosinophils, IL-3 enhances thrombopoiesis, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests additional immunoregulatory roles. Many IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is a species-specific, variably glycosylated cytokine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.