Thermo Fisher Scientific IL-3 beta Polyclonal Antibody, Biotin, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

ELISA (ELISA)

0.25-1.0 µg/mL

-

Product Specifications

Species Reactivity

Rat

Host/Isotype

Goat

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Rat IL-3beta

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929658

Product Specific Information

AA Sequence of recombinant protein: MISDRGSDAH HLLRTLDCRT IALEILVKLP YPQVSGLNNS DDKANLRNST LRRVNLDEFL KSQEEFDSQD TTDIKSKLQK LKCCIPAAAS DSVLPGVYNK DLDDFKKKLR FYVIHLKDLQ PVSVSRPPQP TSSSDNFRPM TVEC.

Preparation: Produced from sera of goats immunized with highly pure Recombinant Rat IL-3beta. Anti-Rat IL-3beta-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Rat IL-3beta by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat IL-3beta (500-P177G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat IL-3beta.

Western Blot: To detect Rat IL-3beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat IL-3beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P177GBT-1MG will be provided as 2 x 500 µg

Target Information

IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoiesis, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is a species-specific, variably glycosylated cytokine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.