Thermo Fisher Scientific Robo1/2 Polyclonal Antibody

Applications and Tested Dilution

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Target Information

Specialized cells at the midline, which separates the left and right halves of the CNS, have roles in directing growth cone behavior. In vertebrate spinal cord, insect ventral nerve cord, and C. elegans, midline cells produce guidance cues such as nectins and slit, which act as attractants and repellents, respectively. These cells may act as gatekeepers to prevent axons from crossing the midline and to induce a switch in growth cone responsiveness to guidance cues beyond the gateway.

Robo is an axon guidance receptor that defines a subfamily of Ig superfamily proteins conserved from fruit flies to mammals. Robo acts as a receptor for the repellent Slit and functions in a cell-autonomous fashion. Non-crossing axons express high levels of Robo, whereas crossing axons express low levels before reaching the midline and high levels after they cross.

Robo1 and Robo2 are human homologs of the Drosophila protein Roundabout. Robo1 is homologous to the C. elegans gene sax3, and Robo2 is homologous to the zebrafish gene astray.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.