Thermo Fisher Scientific PKM2 Recombinant Rabbit Monoclonal Antibody (7B2)
PKM2 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합합니다. 높은 특이성과 재현성을 제공하며, 인간 및 생쥐 시료에 반응합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:20–1:200 |
| Flow Cytometry (Flow) | 1:20–1:200 |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | 1:200–1:1,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 7B2 |
| Immunogen | A synthesized peptide derived from human PKM2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_3093685 |
Target Information
PKM2 (Pyruvate Kinase M2) is an enzyme that catalyzes the conversion of phosphoenolpyruvate to pyruvate and ATP. It interacts with thyroid hormone and may mediate metabolic effects induced by these hormones. PKM2 also binds to the Opa protein, a bacterial membrane protein involved in gonococcal adherence and invasion of human cells, indicating a potential role in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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