Thermo Fisher Scientific Argonaute 3 Recombinant Rabbit Monoclonal Antibody (JJ201-07)
Argonaute 3 단백질을 표적하는 재조합 토끼 단클론 항체로, WB, IHC, ICC/IF, Flow Cytometry에 적합합니다. Human, Mouse, Rat 반응성. HEK293 세포 발현, Protein A 정제. RNAi 관련 연구에 유용하며, 세포질 및 핵에서 발현 확인 가능합니다.
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Thermo Fisher Scientific Argonaute 3 Recombinant Rabbit Monoclonal Antibody (JJ201-07)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JJ201-07 |
| Immunogen | Recombinant protein within human EIF2C3 / Argonaute 3 (aa 689–860) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2911597 |
Product Specific Information
- Positive Control: N2A, SHG-44, F9, NCCIT, rat testis tissue, rat spinal cord tissue, mouse brain tissue, rat brain tissue
- Subcellular Location: Cytoplasm, Nucleus
Target Information
AGO3 (Argonaute-3) is required for RNA-mediated gene silencing (RNAi). It binds short RNAs such as microRNAs (miRNAs) and represses translation of complementary mRNAs.
AGO3 is proposed to stabilize small RNA derivatives (riRNA) from RNA polymerase III-transcribed Alu repeats containing DR2 retinoic acid response elements (RARE) in stem cells, and mediate riRNA-dependent degradation of certain RNA polymerase II-transcribed mRNAs via recruitment of an mRNA decapping complex involving EDC4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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(이미지 파일: MA5-42456_Argonaute_3_Q9H9G7-1_Rabbit.svg, MA5-42456_Argonaute_3_Q9H9G7-1_Rabbit_PDP.jpeg)
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