Thermo Fisher Scientific Argonaute 3 Recombinant Rabbit Monoclonal Antibody (JJ201-07)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Flow Cytometry (Flow)

1:50-1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JJ201-07

Immunogen

Recombinant protein within human eif2c3/ argonaute 3 aa 689-860 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Argonaute 3,uniProtId:Q9H9G7-1,ncbiNodeId:9606,antigenRange:689-860,antigenLength:860,antigenImageFileName:MA5-42456_Argonaute_3_Q9H9G7-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42456_Argonaute_3_Q9H9G7-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2911597

Product Specific Information

Positive Control: N2A, SHG-44, F9, NCCIT, rat testis tissue, rat spinal cord tissue, mouse brain tissue, rat brain tissue.

Subcellular Location: Cytoplasm,Nucleus.

Target Information

AGO3 (protein argonaute-3) is required to RNA-mediated gene silencing (RNAi). It binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. AGO3 is proposed to be involved in stabilization of small RNA derivates (riRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent riRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.