Thermo Fisher Scientific RPA4 Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human RPA4. Recombinant protein control fragment (Product #RP-103779). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RPA4,uniProtId:Q13156-1,ncbiNodeId:9606,antigenRange:1-74,antigenLength:261,antigenImageFileName:PA5-64256_RPA4_Q13156-1_Rabbit.svg,antigenImageFileNamePDP:PA5-64256_RPA4_Q13156-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2646706

Product Specific Information

Immunogen sequence: MSKSGFGSYG SISAADGASG GSDQLCERDA TPAIKTQRPK VRIQDVVPCN VNQLLSSTVF DPVFKVRGII VSQV

Highest antigen sequence identity to the following orthologs: Mouse - 38%, Rat - 41%.

Target Information

The single-stranded-DNA-binding proteins (SSBs) are essential for DNA function in prokaryotic and eukaryotic cells, mitochondria, phages and viruses. Replication protein A (RPA), a highly conserved eukaryotic protein, is a heterotrimeric SSB consisting of three subunits that play an important role in DNA replication, recombination and repair. RPA is one of the major damage- recognition structures involved in the early stage of nucleotide excision repair and may function in telomere maintenance. The binding of human RPA (hRPA) to DNA involves molecular polarity, in which initial hRPA binding occurs on the 5side of a ssDNA substrate and then extends in the 3 direction to create a stably bound hRPA. Widely expressed at low to intermediate levels, RPA 34 kDa subunit, which is also known as RPA4 (replication factor A protein 4), contains 261 amino acids, localizes to nucleus and is preferentially expressed in mucosa of colon and placenta.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.