Thermo Fisher Scientific Nucleolin Monoclonal Antibody (4i51)
Thermo Fisher의 Nucleolin 단클론 항체(4i51)는 인간 및 비인간 영장류 시료에서 Nucleolin 단백질 검출용으로 적합합니다. Western blot, ICC/IF, ELISA, IP 등 다양한 응용에 사용 가능하며, HeLa 세포를 양성 대조군으로 권장합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 | |
| Immunocytochemistry (ICC/IF) | 1:10–1:100 | |
| ELISA | Assay-dependent | |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Non-human primate |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4i51 |
| Immunogen | Nuclear matrix extract from human MDCK cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Tissue culture supernatant with 30% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_795066 |
Product Specific Information
MA1-25778 will not react with mouse or rat.
Suggested positive controls for this product are HeLa nuclear extract or HeLa cells.
Target Information
Nucleolin (NCL) is a eukaryotic nucleolar phosphoprotein involved in the synthesis and maturation of ribosomes. It is mainly located in dense fibrillar regions of the nucleolus. The human NCL gene consists of 14 exons and 13 introns, spanning approximately 11 kb. Intron 11 encodes a small nucleolar RNA termed U20.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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