Thermo Fisher Scientific Ninein Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:50

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human NIN. Recombinant protein control fragment (Product #RP-102160). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Ninein,uniProtId:Q8N4C6-1,ncbiNodeId:9606,antigenRange:530-665,antigenLength:2090,antigenImageFileName:PA5-82224_Ninein_Q8N4C6-1_Rabbit.svg,antigenImageFileNamePDP:PA5-82224_Ninein_Q8N4C6-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.10 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2789385

Product Specific Information

Immunogen sequence: EERLTQMRNE YERQCRVLQD QVDELQSELE EYRAQGRVLR LPLKNSPSEE VEANSGGIEP EHGLGSEECN PLNMSIEAEL VIEQMKEQHH RDICCLRLEL EDKVRHYEKQ LDETVVSCKK AQENMKQRHE NETHTL

Target Information

Ninein is a component of subdistal appendages of the mother centriole, localized specifically in the pericentriolar matrix of the centrosome. It may be involved in microtubule minus-end capping, centriole positioning, and protein anchoring. Recent studies have shown that the microtubule nucleation and anchoring at the centrosome are independent processes linked by ninein function. At least five human ninein isoforms with divergent C-terminal domains and two mouse isoforms have been reported. Data indicates that the combined action of domains (C-terminal and N-terminal) might, in the absence of coiled coil region, be sufficient to localize ninein to the mother centriole. Microinjection of antibodies against ninein into metaphase HeLa cells can disrupt the reformation of tubular conformation of proteins within the centrosome following cell division, and consequently lead to dispersal of centrosomal material throughout the cytosol. MTOC (microtubule organizing center) function can be disrupted when anti-ninein antibodies are injected into postmitotic cells. Antibodies against ninein are present in sera from patients with autoimmune diseases that develop autoantibodies to centrosomal proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.