Thermo Fisher Scientific PTPN12 Polyclonal Antibody
Rabbit polyclonal antibody against human PTPN12. Validated for WB, IHC, IHC-P, and IP applications. Recommended dilutions range from 1:100 to 1:10,000. Supplied as a liquid, 0.2 mg/mL, stored at 4°C. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 710 and 760 of human protein tyrosine phosphatase, non-receptor type 12 (PTPN12, UniProt ID: Q05209-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
PTP PEST is an 88 kDa cytosolic protein tyrosine phosphatase ubiquitously expressed in mammalian tissues. It exhibits high specific activity in vitro with artificial tyrosine-phosphorylated substrates. PTP PEST is regulated via phosphorylation of serine 39, catalyzed by both protein kinase C and protein kinase A.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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