Thermo Fisher Scientific POLR3A Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

Immunoprecipitation (IP)

1:100-1:500

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 182 and 482 of POLR3A (Uniprot ID#O14802) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:POLR3A,uniProtId:O14802-1,ncbiNodeId:9606,antigenRange:182-482,antigenLength:1390,antigenImageFileName:PA5-21730_POLR3A_O14802-1_Rabbit.svg,antigenImageFileNamePDP:PA5-21730_POLR3A_O14802-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.43 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_11152880

Product Specific Information

Recommended positive controls: IMR32, SK-N-AS.

Predicted reactivity: Mouse (97%), Zebrafish (94%), Xenopus laevis (94%), Chicken (97%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR3A is the largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. It forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.