Thermo Fisher Scientific POLR3A Polyclonal Antibody
POLR3A 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, ICC/IF, IP에 사용 가능. 항원 친화 크로마토그래피로 정제된 액상 제품으로 높은 특이성과 안정성 제공. 연구용으로 DNA-dependent RNA polymerase III 관련 연구에 적합.
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Thermo Fisher Scientific POLR3A Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 182–482 of POLR3A (Uniprot ID: O14802) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.43 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11152880 |
Product Specific Information
- Recommended positive controls: IMR32, SK-N-AS
- Predicted reactivity: Mouse (97%), Zebrafish (94%), Xenopus laevis (94%), Chicken (97%), Bovine (98%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR3A is the largest and catalytic core component of RNA polymerase III, which synthesizes small RNAs such as 5S rRNA and tRNAs. It forms the polymerase active center together with the second largest subunit. The single-stranded DNA template strand is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site, promoting translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site via conformational switching.
Usage Notice
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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