Thermo Fisher Scientific Clostridium tetani Tetanus Toxin VHH-8His-Cys-tag Recombinant Alpaca Monoclonal Antibody (SAA1236)

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Surface plasmon resonance (SPR)

Assay-dependent

-

Product Specifications

Species Reactivity

Bacteria

Host/Isotype

Alpaca / VHH

Expression System

CHO cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SAA1236

Immunogen

Recombinant Clostridium tetani tetX/Tetanus toxin.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Metal-chelate chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Product Specific Information

Compatible with Direct ELISA

Target Information

Tetanus toxin is a neurotoxin produced by the vegetative spore of Clostridium tetani. The toxin causes tetanus, a disease that is characterised by prolonged contraction of skeletal muscle. World-wide, approximately 1 million cases of tetanus occurs annually, resulting in approximately 300,000 to 500,000 deaths, mostly in developing countries. The tetanus toxin has a molecular weight of approximately 150kDa and consists of a heavy chain (B-chain, 100kDa) and a light chain (A-chain, 50kDa) that are connected by a disulfide bond. The toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits the release of the neurotransmitters GABA and glycine by synaptobrevin-2. This results in overactivity in the muscle. The binding of the toxin to neurons is irreversible.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.