Thermo Fisher Scientific Eukaryotic 18S rRNA Endogenous Control (VIC/MGB probe, primer limited)

Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (VIC™/MGB Probe, Primer Limited)

The Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (VIC™/MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. The probe is labeled with VIC™ dye - MGB, and the primers are limited. This control can be used for multiplex or singleplex PCR reactions. It is compatible with Inventoried and Non-Inventoried TaqMan™ Gene Expression Assays, Custom Gene Expression Assays, and/or Custom TaqMan™ Primers and Probes.

Assay Details

TaqMan™ Endogenous Controls

TaqMan™ Endogenous Controls eliminate months of assay design, formulation, and testing by providing pre-designed probe and primer sets to normalize RNA or DNA amounts in reactions.

Complete Solution for Quantitative Gene Expression

TaqMan™ Endogenous Controls simplify the selection of appropriate controls for quantifying gene expression in human, mouse, rat, and eukaryotic systems, minimizing trial-and-error in assay setup.

Simple to Use

All components are QC tested, formulated into a single 20X mix, and functionally validated. Compatible with universal two-step RT-PCR conditions. Simply add TaqMan™ Universal PCR Master Mix (with or without AmpErase™ UNG) and your cDNA sample to generate sensitive, reproducible, and quantitative gene expression data on Applied Biosystems instruments such as:

• 7900HT
• 7300
• 7500 Real-Time PCR Systems
• 7000 and 7700 Sequence Detection Systems

Flexible Offering

Each endogenous control uses proven 5′ nuclease chemistry. Available configurations include:

• FAM™ dye-labeled TaqMan™ MGB probe (250 nM) with two unlabeled PCR primers (900 nM each)
• VIC™ dye-labeled TaqMan™ MGB probe (250 nM) with two unlabeled PCR primers (150 nM each; primer limited)
• VIC™ dye-labeled TAMRA™ probe (250 nM) with two unlabeled PCR primers (150 nM each; primer limited)

Choosing the Right Endogenous Control

Endogenous controls normalize target gene expression by correcting for cDNA input differences. The control should exhibit consistent expression across all samples. For multiplexing, ensure the control gene is expressed at higher levels than the target gene.

Multiplex vs. Singleplex PCR

TaqMan™ Endogenous Controls with VIC™ reporter dye are primer limited, enabling multiplexing with target assays when the control gene is more abundant. Controls with FAM™ dye are not primer limited and are intended for singleplex reactions.

For Research Use Only. Not for use in diagnostic procedures.

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