Thermo Fisher Scientific CDKN2B Monoclonal Antibody (OTI8H4), TrueMAB
CDKN2B 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot, IHC, ICC 등 다양한 응용에 적합합니다. HEK293T에서 생산된 full-length human recombinant protein을 면역원으로 사용하였으며, carrier-free 형태로 제공되어 conjugation 실험에 유용합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI8H4 |
| Immunogen | Full length human recombinant protein of human CDKN2B produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The four known 15–19 kDa INK4 proteins (p15INK4b, p16INK4a, p18INK4c, and p19INK4d) specifically bind and inhibit CDK4 and CDK6 but not other CDKs such as cyclin E-Cdk2, cyclin A-Cdk2, or cyclin B-Cdk1.
INK4 genes show distinct tissue-specific expression patterns, indicating non-redundant functions.
p18INK4c is expressed during the G1 to S transition in the eukaryotic cell cycle and is maximally induced as cells enter S-phase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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