Thermo Fisher Scientific Claudin 3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 109 publications 109 publications

Immunohistochemistry (IHC)

-

View 64 publications 64 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

View 12 publications 12 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 10 publications 10 publications

Immunocytochemistry (ICC/IF)

1:100

View 69 publications 69 publications

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

-

View 2 publications 2 publications

in situ PLA (PLA)

-

View 1 publication 1 publication

Immunoelectrophoresis (IE)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 11 publications 11 publications

Product Specifications

Species Reactivity

Dog, Human, Mouse

Published species

Amphibian, Bovine, Dog, Fish, Goat, Hamster, Human, Mouse, Pig, Plant, Rabbit, Rat, Rhesus monkey, Sheep, Virus, Xenopus, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the C-terminal region of mouse Claudin-3 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Claudin 3,uniProtId:Q9Z0G9-1,ncbiNodeId:10090,antigenRange:219,antigenLength:219,antigenImageFileName:34-1700_Claudin_3_Q9Z0G9-1_Rabbit.svg,antigenImageFileNamePDP:34-1700_Claudin_3_Q9Z0G9-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533158

Product Specific Information

This antibody is specific for the 22 kDa Claudin-3 protein. Reactivity has been confirmed with mouse liver and lung homogenates, canine MDCK and human MCF-7 cell lysates by western blotting, and with formalin-fixed, paraffin-embedded (FFPE) human normal colon and colon cancer tissues by immunohistochemistry. For best results in immunohistochemistry with formalin-fixed, paraffin-embedded (FFPE) tissues, heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0, is required prior to staining.

Target Information

Claudin 1-8 proteins are a family of transmembrane proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact; made up of network of strands to act as a molecular gasket for preventing the leakage of ions, water etc. between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition. Differential expression of this gene has been observed in different types of malignancies, including breast cancer, ovarian cancer, hepatocellular carcinomas, urinary tumors, prostate cancer, lung cancer, head and neck cancers, thyroid carcinomas, etc. Alternatively spliced transcript variants encoding different isoforms have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.