Thermo Fisher Scientific TNFA Monoclonal Antibody (OTI3H10), TrueMAB

Applications

• Western Blot (WB)
  Tested Dilution: 1:500

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

TNF alpha is a multifunctional proinflammatory cytokine belonging to the tumor necrosis factor (TNF) superfamily.
It is mainly secreted by macrophages and binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR receptors.
TNF alpha regulates immune cell activity, proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.
It forms multimers but shows a single 17 kDa band under non-reducing SDS-PAGE conditions.
Knockout mouse studies indicate neuroprotective roles, and TNF alpha can induce tumor necrosis in vivo.
It also contributes to immune responses against bacterial, viral, parasitic, and fungal infections and is implicated in autoimmune diseases, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.