Thermo Fisher Scientific RHOA Monoclonal Antibody (1C10)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

-

Immunocytochemistry (ICC/IF)

10 µg/mL

-

ELISA (ELISA)

10 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, lambda

Class

Monoclonal

Type

Antibody

Clone

1C10

Immunogen

RHOA (AAH01360, 1 a.a. approximately 193 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RhoA,uniProtId:P61586-1,ncbiNodeId:9606,antigenRange:1-193,antigenLength:193,antigenImageFileName:H00000387-M06_RhoA_P61586-1_House_mouse.svg,antigenImageFileNamePDP:H00000387-M06_RhoA_P61586-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: MAAIRKKLVI VGDGACGKTC LLIVFSKDQF PEVYVPTVFE NYVADIEVDG KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSIDSPD SLENIPEKWT PEVKHFCPNV PIILVGNKKD LRNDEHTRRE LAKMKQEPVK PEEGRDMANR IGAFGYMECS AKTKDGVREV FEMATRAALQ ARRGKKKSGC LVL

Target Information

RhoA regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. It is involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis, and plays an essential role in cleavage furrow formation. RhoA is required for the apical junction formation of keratinocyte cell-cell adhesion, and serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RhoA stimulates PKN2 kinase activity and may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP. It is essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.