Thermo Fisher Scientific Adenylate Kinase 1 Polyclonal Antibody
Rabbit polyclonal antibody targeting Adenylate Kinase 1 (AK1). Suitable for WB and IHC applications. Reacts with human and mouse samples. Validated positive controls include A549, HCT116, and mouse brown adipose tissue. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1–188 of Human Adenylate Kinase 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.4 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545830 |
Product Specific Information
- Recommended positive controls: A549, HCT116, mouse brown adipose tissue
- Predicted reactivity: Mouse (89%), Rat (90%), Xenopus laevis (80%), Dog (97%), Chicken (86%), Sheep (96%), Bovine (96%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Adenylate kinase is an enzyme that regulates the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates: AK1, AK2, and AK3.
- AK1: Found in the cytosol of skeletal muscle, brain, and erythrocytes
- AK2 / AK3: Found in the mitochondria of other tissues including liver and heart
AK1 has been associated with a rare genetic disorder causing nonspherocytic hemolytic anemia due to mutations in the AK1 gene that reduce the enzyme’s catalytic activity.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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